The Harbin Vet Institute has recently developed two specific processes to test cow tuberculosis pathogen. It only takes three hours for the process to roll out test results of the DNA of M.vaccae at 50pg. The new PCR process selects an esat-6 gene that is specifically possessed by the tuberculosis mycobacterium compound, in an attempt to increase the target genes for PCR. The further specificity and sensitivity analysis results in an esat-6 gene based PCR test process. The new process is able to perform fast test with high sensitivity and specificity, with both of them reaching the same level of overseas counterpart products. Another process, named ELISA test kit, integrates a number of highly specific antigens in E. coli. In addition to activating an efficient gene expression, it produces an antigen for diagnosing cow tuberculosis. Clinical trials have shown that the processes can sort out cows affected with tuberculosis in a fast manner, and they are promising for a development perspective with an enhanced specificity.
The test kits can be applied to raw milk samples for testing the DNA of Brucella through nested-PCR. With a test sensitivity ranging from 50 to 100 bacteria/ml milk sample, the processes produce the diagnostic results within 48 hours. The test kits can perform test on diverse samples, including milk, fetus stomach fluid from miscarriage, vagina secretions, serum, and embryo membrane. With a strong specificity and desirable repetition capacity, the test kits can be preserved for 6 months at a temperature of -20º C. |
